Selected issues of anaerobic digestion based on the studies on hydrogen- and methane yielding bioreactors


  • Anna Sikora Institute of Biochemistry and Biophysics Polish Academy of Sciences
  • Anna Detman Institute of Biochemistry and Biophysics Polish Academy of Sciences
  • Aleksandra Chojnacka Institute of Biochemistry and Biophysics Polish Academy of Sciences
  • Damian Mielecki Institute of Biochemistry and Biophysics Polish Academy of Sciences


Słowa kluczowe:

microorganisms, biohydrogen, methane, anaerobic conditions, metabolism


Anaerobic digestion of organic matter results from the metabolic activity of many groups of microorganisms. Interactions between microorganisms during acidogenesis, acetogenesis and methanogenesis, source of inoculum, type of feedstock and operational conditions determine metabolic pathways in bioreactors and consequently the efficiency of fermentation processes. In innovative installations it is desirable to separate acidogenesis from acetogenesis and methanogenesis to favour respectively the production of biohydrogen or biomethane under controlled conditions.


Sieber, J.R., McInerney, M.J., Gunsalus, R.P. (2012). Genomic Insights into Syntrophy: The Paradigm for Anaerobic Metabolic Cooperation. Annu. Rev. Microbiol., 66, 429–452.

Mao, C.L., Feng, Y.Z., Wang, X.J., Ren, G.X. (2015). Review on research achievements of biogas from anaerobic digestion. Renew. Sust. Energ. Rev., 45, 540–555.

Sikora, A., Detman, A., Chojnacka, A., Błaszczyk, M.K. (2017). Anaerobic Digestion: I. A Common Process Ensuring Energy Flow and the Circulation of Matter in Ecosystems. II. A Tool for the Production of Gaseous Biofuels. In A.F. Jozala (ed.), Fermentation Processes (pp. 271–301). Rijeka: InTech.

Thauer, R.K., Kaster, A.K., Seedorf, H., Buckel, W., Hedderich, R. (2008). Methanogenic Archaea: ecologically relevant differences in energy conservation. Nature Reviews Microbiology, 6, 579–591.

Schievano, A., Tenca, A., Lonati, S., Manzini, E., Adani, F. (2014). Can two-stage instead of one-stage anaerobic digestion really increase energy recovery from biomass? Applied Energy, 124, 335–342.

Sen, B., Aravind, J., Kanmani, P., Lay, C.-H. (2016). State of the art and future concept of food waste fermentation to bioenergy. Renewable and Sustainable Energy Reviews, 53, 547–557.

Jarunglumlert, T., Prommuak, C., Putmai, N., Pavasant, P. (2018). Scaling-up bio-hydrogen production from food waste: Feasibilities and challenges. Int. J. Hydrogen Energy, 43, 634–648.

Guwy, A.J., Dinsdale, R.M., Kim, J.R., Massanet-Nicolau, J., Premier, G. (2011). Fermentative biohydrogen production systems integration. Bioresource Technology, 102, 8534–8542.

Kleinsteuber, S. (2018). Metagenomics of methanogenic communities in anaerobic digesters. Biogenesis of hydrocarbons. In A.J.M. Stams, D.Z. Sousa (eds.), Biogenesis of Hydrocarbons, Handbook of Hydrocarbon and Lipid Microbiology (pp. 1–23). Springer International Publishing AG.

Cai, M., Wilkins, D., Chen, J., Ng, S.-K., Lu, H., Jia, Y., Lee, P.K.H. (2016). Metagenomic reconstruction of key anaerobic digestion pathways in municipal sludge and industrial wastewater biogas-producing systems. Front. Microbiol., 7, 778.

Campanaro, S., Treu, L., Kougias, P.G., De Francisci, D., Valle, G., Angelidaki, I. (2016). Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy. Biotechnology for Biofuels, 9, 26.

Guo, J., Peng Y., Ni, B.-J., Han, X., Fan, L., Yuan, Z. (2015). Dissecting microbial community structure and methane-producing pathways of a full-scale anaerobic reactor digesting activated sludge from wastewater treatment by metagenomics sequencing. Microbial Cell Factories, 14, 33.

Detman, A., Mielecki, D., Pleśniak, Ł., Bucha, M., Janiga, M., Matyasik, I., Chojnacka, A., Jędrysek, M.-O., Błaszczyk, M.K., Sikora, A. (2018). Methane-yielding microbial communities processing lactate-rich substrates — a piece of the anaerobic digestion puzzle. Biotechnology for Biofuels, 11, 116.

Detman, A., Chojnacka, A., Mielecki, D., Błaszczyk, M.K., Sikora, A. (2018). Inhibition of hydrogen-yielding dark fermentation by ascomycetous yeasts. Int. J. Hydrogen Energy, 43, 10967–10979.



Jak cytować

Sikora, A., Detman, A., Chojnacka, A., & Mielecki, D. (2019). Selected issues of anaerobic digestion based on the studies on hydrogen- and methane yielding bioreactors. Eco-Energetics: Technologies, Environment, Law and Economy, 2, 129–134.